One of the activities we did involved extracting DNA from strawberries.
This recipe is enough for a group of 50 students. Gently add 50 milliliters of shampoo. Place a strawberry in a Ziploc bag and gently mash for two minutes, being careful not to pop the bag. Use a pipette to add 10 milliliters of the DNA extraction buffer to the bag and mash for one minute.
Assemble the DNA filter by draping a coffee filter over Dna extraction strawberries plastic cup. Make sure the bottom of the filter does not touch the bottom of the cup, and the top of the filter is folded over the cup to hold it in place.
Pour the strawberry mixture into the coffee filter and let it drain for five to 10 minutes. Tilt the tube and pour an equal amount of cold isopropyl alcohol into the test tube.
The DNA will precipitate to the top of the solution and will resemble a white, fluffy cloud.
DNA extraction is the process of obtaining pure DNA from a sample, either from living or non-living cells, such as those found in viruses. This technique is commonly used in the medical field, where early detection of diseases and disorders significantly increases the survival rates of afflicted individuals. The salt makes the DNA molecules stick together, and separate from the proteins that are also released from the cells. 3. Place the funnel lined with gauze into the test tube. 4. Pour the strawberry DNA extraction buffer mixture into the gauze and filter the mixture into the tube through the gauze. Martinez 1. Torii Martinez Mrs. Campbell Acc. Biology B 22 March Strawberry DNA Extraction Lab Report Introduction: In this lab my group and I are testing what DNA looks like.
The shampoo helps dissolve the fatty cell membrane, while the salt makes the DNA molecules stick together and breaks up the proteins that bind to the DNA. The alcohol causes the DNA to precipitate, or rise to the top of the solution.
Extensions Try to extract DNA from other fruits and vegetables like grapes, kiwi, tomatoes, bananas or lettuce. Which fruit or vegetable produces the most DNA?
Try different types of shampoo and dish soap and compare the results. Background information Cells are the functional and structural unit for life in both plants and animals. There are pretty obvious differences between the two, but also many similarities.
Both have many of the same organelles, including a nucleus, which is the control for the cell. The nucleus contains DNA, or deoxyribonucleic acid. In both plants and animals, the DNA is in the same shape - the famous double helix - that resembles a spiral staircase.
DNA molecules in plant cells and animal cells are made from the same four chemical building blocks: The way the nucleotides are arranged and the information they encode determines whether the organism will grow fur, scales, leaves or skin.DNA Extraction from Fruit (Adapted from Isolation of DNA from Onion—Woodrow Wilson ) Loretta Loykasek, Burleson High School, Burleson, TX mango and strawberry have been found to yield the most DNA.
Cut a small piece of fruit, peel any tough skin and take out large seeds. Cut into small pieces. 5. Place fruit in blender and pour. Extracting DNA from a Banana and Other Fruits. Introduction.
All living things, bananas and people included, pass on information from one generation to the next using the same basic material, urbanagricultureinitiative.com every living organism, most cells contain a complete set of DNA instructions.
Create the DNA extraction buffer by mixing milliliters of water with two teaspoons of salt. Gently add 50 milliliters of shampoo. Place a strawberry in a Ziploc bag and gently mash for two minutes, being careful not to pop the bag.
Lab: DNA EXTRACTION LESSON SUMMARY In this lesson, students will remove DNA from plant cells (strawberries) and compare the process to DNA extraction in an actual biotech lab (will discuss in lecture).
DNA is important because it is the chemical code that instructs each cell’s activity. Published: Wed, 23 May INTRODUCTION This life science based experiment will test strawberries in their various stages of ripeness, in order to see which stage will yield the most extractable DNA.
An extraction kit will be designed from common household items, such as salt and detergent, in order to purify the DNA so that it is visible to the naked eye and can be weighed.
The DNA was dissolved in the water contained in the sports drink.
DNA does not dissolve in alcohol. When the cold alcohol was layered on top of the cheek cell solution the DNA precipitated out of.